Effect of Eucalyptus globulus essential oil supplementation on advanced motility parameters and DNA integrity of human sperm.

Modou M. Mbaye,^1,2,3 Bouchra El Khalfi,¹ Achraf Zakaria,² Noureddine Louanjli ^2,3 Mustafa Zakaria⁴ and Abdelaziz Soukri¹

Abstract:

Reduced sperm motility remains one of the most important causes of male infertility. Many reports have indicated that treatment of subnormal semen samples with certain agents (pentoxifylline, relaxin, prostaglandin E, diltiazem etc.) prior to artificial insemination significantly improves the fertilization potential of spermatozoa. In our previous work, it has been shown that the essential oil of Eucalyptus globulus has interesting biological properties on the motility and vitality of human spermatozoa. Therefore, in this study, we aimed to verify the effect of Eucalyptus globulus essential oil supplementation on advanced motility parameters and DNA integrity of 25 semen samples from male volunteers diagnosed as asthenozoospermic.

Our results showed that eucalyptus globulus essential oil, over an incubation period of 5 to 10 minutes of exposure at 37°C under 5% CO2, very significantly improved advanced motility parameters: curvilinear velocity (VLC), linear velocity (VSL), average path velocity (VAP), and amplitude of movement (ALH), with the effect of increasing VCL decreasing linearity (LIN), mean line (STR), and mean oscillation (WOB).

Eucalyptus globulus essential oil at 5 minutes had no significant effect on DNA fragmentation index (DFI) and chromatin decondensation index (SDI).

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